Genome sequencing using long-read sequencing
نویسندگان
چکیده
منابع مشابه
Reconstructing complex regions of genomes using long-read sequencing technology.
Obtaining high-quality sequence continuity of complex regions of recent segmental duplication remains one of the major challenges of finishing genome assemblies. In the human and mouse genomes, this was achieved by targeting large-insert clones using costly and laborious capillary-based sequencing approaches. Sanger shotgun sequencing of clone inserts, however, has now been largely abandoned, l...
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The 22-gigabase genome of loblolly pine (Pinus taeda) is one of the largest ever sequenced. The draft assembly published in 2014 was built entirely from short Illumina reads, with lengths ranging from 100 to 250 base pairs (bp). The assembly was quite fragmented, containing over 11 million contigs whose weighted average (N50) size was 8206 bp. To improve this result, we generated approximately ...
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BACKGROUND Recent studies on genome assembly from short-read sequencing data reported the limitation of this technology to reconstruct the entire genome even at very high depth coverage. We investigated the limitation from the perspective of information theory to evaluate the effect of repeats on short-read genome assembly using idealized (error-free) reads at different lengths. METHODOLOGY/P...
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ژورنال
عنوان ژورنال: Revista de la Academia Colombiana de ciencias exactas, físicas y naturales
سال: 2023
ISSN: ['0370-3908', '2382-4980']
DOI: https://doi.org/10.18257/raccefyn.1937